Journal: Frontiers in Immunology

Article Title: Intranasal MMI-0100 Attenuates Aβ 1−42 - and LPS-Induced Neuroinflammation and Memory Impairments via the MK2 Signaling Pathway

doi: 10.3389/fimmu.2019.02707

Figure Lengend Snippet: Information on antibodies used in this experiment.

Article Snippet: Anti-p-MK2 Rabbit mAb Anti-t-MK2 Rabbit mAb Anti-p-p38 Rabbit mAb , 1:1,000 1:1,000 1:1,000 , 3700S D16188-0025 4511S , CST, USA BBI Life Science CST, USA.

Techniques: Concentration Assay

Journal: Frontiers in Immunology

Article Title: Intranasal MMI-0100 Attenuates Aβ 1−42 - and LPS-Induced Neuroinflammation and Memory Impairments via the MK2 Signaling Pathway

doi: 10.3389/fimmu.2019.02707

Figure Lengend Snippet: Effect of MMI-0100 on Aβ 1−42 - or LPS-induced activation of MAPK in the hippocampus in mice. The upper panel is a representative immunoblot of pERK, tERK, pJNK, tJNK, p38, and p-p38 detected by Western blotting; the lower panel is quantification of MAPK signaling pathway-related protein expressed as the ratio of pERK/tERK, pJNK/tJNK, and p-p38/p38. Values were expressed as mean ± SEM, n = 5 in each group. ** p < 0.01 and *** p < 0.001 compared with vehicle group.

Article Snippet: Anti-p-MK2 Rabbit mAb Anti-t-MK2 Rabbit mAb Anti-p-p38 Rabbit mAb , 1:1,000 1:1,000 1:1,000 , 3700S D16188-0025 4511S , CST, USA BBI Life Science CST, USA.

Techniques: Activation Assay, Western Blot

Journal: Frontiers in Immunology

Article Title: Intranasal MMI-0100 Attenuates Aβ 1−42 - and LPS-Induced Neuroinflammation and Memory Impairments via the MK2 Signaling Pathway

doi: 10.3389/fimmu.2019.02707

Figure Lengend Snippet: Effects of MMI-0100 treatment on LPS-induced inflammatory response and the expression of pMK2 in BV-2 cells. (A) MMI-0100 reduces LPS (1 μg/ml)-induced release of pro-inflammatory mediators such as IL-6, IL-1β, TNF-α, COX-2, and iNOS level in BV-2 cells. (B) The upper panel is a representative immunoblot of pMK2, tMK2, and GAPDH detected by Western blotting; the lower panel is quantification of MK2 expressed as the ratio of pMK2/tMK2. ** p < 0.01 compared with vehicle group vs LPS group. # p < 0.05 and ## p < 0.01 compared with the LPS group vs. LPS + MMI-0100 (10 −5 , 10 −6 , 10 −7 , and 10 −8 M) at different doses. Values were expressed as mean ± SEM.

Article Snippet: Anti-p-MK2 Rabbit mAb Anti-t-MK2 Rabbit mAb Anti-p-p38 Rabbit mAb , 1:1,000 1:1,000 1:1,000 , 3700S D16188-0025 4511S , CST, USA BBI Life Science CST, USA.

Techniques: Expressing, Western Blot